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. 2010 Feb 25;29(7):1248–1261. doi: 10.1038/emboj.2010.21

Figure 10.

Figure 10

Tautomycin abolishes CGG and Sam68 aggregate formation. (A) COS7 cells were co-transfected with a plasmid expressing 60 CGG repeats and either treated with solvent (a) or 1 μM of tautomycin (b), then analysed by FISH/IF 24 h after transfection. (B) The percentage of cells containing CGG RNA aggregates in transfected COS7 cells treated with solvent, 0.3 or 1 μM of tautomycin. (C) Similar to panel A but followed by RNA extraction 24 h after transfection and qRT–PCR quantification of the CGG repeats RNA and of the neomycin mRNA. CGG and neomycin cassettes are expressed from the same pcDNA3.1 plasmid, but under different promoters. (D) Similar to panel A or C but COS7 cells were also co-transfected with an ATP11B minigene. Inclusion of exon-28B was identified 24 h after transfection by RNA extraction followed by RT–PCR.