Figure 1.

H1⁰ contains three important binding regions: sites S1 and S2 within the central globular domain along with the unstructured C-terminal domain. To determine how these regions cooperatively bind, we examined eight cell lines with GFP-tagged H1⁰ constructs representing all possible combinations of these three binding regions (involving deletions of the C-terminal tail or alanine substitutions of the key residues shown within S1 or S2). We named constructs based on their functional binding regions and signify them with a bold italic font. Although the pattern of fluorescence varied between constructs (see Supplementary Figures S1 and S6 for details), we performed experiments in homogeneous, euchromatic regions of nuclei to exclude the effects of binding at special sites such as nucleoli or the nuclear periphery. A full-colour version of this figure is available at The EMBO Journal Online.