Figure 3.

FRAP was performed for each of the eight H1⁰ constructs representing all possible combinations of the three key H1⁰ binding regions (S1, S2, and C). For each construct (A–H), a sample fit is shown along with the average bound/free fraction (b/f) obtained from 10–20 such fits taken over 3 or more days. Table I provides the corresponding diffusion coefficients and binding association and residence times. FRAP recoveries from the eight constructs span three orders of magnitude in time (colour coded with increasing time from 0 to 400 s), with the fastest triple-knockout 3KO recovery taking <3 s and the slowest wild-type WT recovery taking over 400 s.