Figure 6.

Cooperativity analysis for the key H1⁰ binding regions. (A) In the left most bar-plot the bound/free fraction in S1C is significantly greater than the sum of bound/free fractions in S1 and C, indicative of significant binding cooperation between the C-terminal tail and the S1 site. Similarly, the middle bar indicates significant binding cooperation between the C-terminal tail and the S2 site. In constrast, the inset shows no significant binding cooperation between the S1 and S2 sites by themselves. (B) Once two regions cooperatively bind, either C-S2 (left bar) or C-S1 (right bar), the remaining site (either S1 or S2) binds with cooperativity an order of magnitude larger than in the initial cooperative binding step.