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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mol Biochem Parasitol. 2010 Mar 23;171(2):89–96. doi: 10.1016/j.molbiopara.2010.03.002

Figure 3. Phosphorylation of Leishmania CRKs with Civ1.

Figure 3

(A) Sequence alignment of L. major CRK1-4, 6-8, S. cerevisiae CDC28, the natural substrate for Civ1, and human CDK2. The T-loop residue is indicated (*) (B) Phosphorylation of L. major CRKs by Civ1-GST. Left panel: purified recombinant histidine-tagged CRK proteins. Right panel: Phosphorylation by Civ1-GST.