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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mol Biochem Parasitol. 2010 Mar 23;171(2):89–96. doi: 10.1016/j.molbiopara.2010.03.002

Figure 4. CRK3:CYCA in L. major.

Figure 4

(A) Western blot of L. major promastigote cell lysates probed with anti-HA antibody. Lane 1: L. major [pXG-CYCA-HA], Lane 2: L. major [pXG-HA-CYCA], Lane 3, wild type L. major (B) Upper panel: Silver stained SDS-PAGE gel of protein eluted from anti-HA antibody affinity column. Lower panel. Western blot with anti-CRK3 antibody. Lane 1: L. major [pXG-HA-CYCA] Lane 2: wild type L. major. (C) histone H1 kinase assay with immuno-precipitated HA-CYCA Lane 1: L. major [pXG-HA-CYCA], Lane 2: wild type L. major. H1; histone H1