Table 1.

Gene	Destination vector	Primer	Primer sequence 5′ to 3′
L. mexicana CRK3	pET28a	OL225	GAATTCCATATGTCTTCGTTTGGCCGTGTGA
L. mexicana CRK3	pET28a	OL894	CTCGAGCTACCAACGAAGGTCGCTGA
L. mexicana CRK3	Mutagenesis primer	OL877	CCCATGCACACCTACGAGCACGAGGTGGTT ACG
L. mexicana CRK3	Mutagenesis primer	OL878	CGTAACCACCTCGTGCTCGTAGGTGTGCAT GGG
L. mexicana CYCA	pET21a	OL813	CATATGGCGGTCCCACTGCGAATG
L. mexicana CYCA	pET21a	OL814	CTCGAGCGCAGAAGTTGAAATGAA
L. major CRK1	pET15b	OL1783	CCATATGACCAGCCGGTACGAGCGGCA GGAGAAGATC
L. major CRK1	pET15b	OL1784	CGGATCCCTAAAACTGGAGGCTAAAGTACG GGTG
L. major CRK2	pET15b	OL1785	CCATATGCGGAGCAGCGGCCCCACCCCAGC GC
L. major CRK2	pET15b	OL1786	CGGATCCTTACGACTGCTGCTGCTGCTGCTG CTG
L. major CRK3	pET15b	OL1787	CCATATGTCTTCGTTTGGCCGTGTTACCGCCC
L. major CRK3	pET15b	OL1788	CGGATCCCTACCAGCGAAGGTCACTGAACC ACGGG
L. major CRK4	pET15b	OL1789	CCATATGTCGACGGCGGGTCGGTACAAGCA CG
L. major CRK4	pET15b	OL1790	CGGATCCTCATAGCAAGTGGCAGGCCTCCA TCGTC
L. major CRK6	pET15b	OL1791	CCATATGTCCGCGTCAGTGAACGACTTGGA TG
L. major CRK6	pET15b	OL1792	CGGATCCCTACGCATCCTTCATAAAGGGGT GTTCC
L. major CRK7	pET15b	OL1793	CCATATGGACAAGTACGCGTTGGGGCCG GTTATC
L. major CRK7	pET15b	OL1794	CGGATCCTCATGCACGCAGCAAGGTATCTG AGAG
L. major CRK8	pET15b	OL1795	CCATATGGGAGGGGAACTGGATAACCAGAAC
L. major CRK8	pET15b	OL1796	CGGATCCTCAATGCTCCAGCTCCTTCCGCTT GACC
L. mexicana CYCA	pXG	OL1935	CCC CGG GAT GGC GGT CCC ACT GCG AAT GAG GA
L. mexicana CYCA	pXG	OL1936	TGG ATC CTC AGG CAT AGT CCG GGA CGT CGT AGG GGT
L. mexicana CYCA	pXG	OL1937	CCC CGG GAT GTA CCC CTA CGA CGT CCC GGA CTA TGC
L. mexicana CYCA	pXG	OL1938	GTG GAT CCT CAC GCA GAA GTT GAA ATG A AA GG

Restriction endonuclease sites are underlined

Mutagenesis sites are in bold