Step	Description
1	Initial denaturation at 94 °C for 5 min
2	Denaturation at 94 °C for 30 s
3	Annealing at 50 °C for 30 s
4	Extension at 72 °C for 30 s
5	Repeat steps 2–4 for N times
6	Additional extension at 72 °C for 5 min