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EC–substratum interaction influences NF-κB-dependent expression of adhesion molecules. Real-time PCR ΔΔCt of (A) VCAM-1 and (B) ICAM in MEEC (opened histogram) and EC-TCPS (closed histogram) stimulated for 6 h with 5 ng/ml TNF-α. Flow cytometry analysis of (C) VCAM and (D) ICAM expression on embedded EC (solid line), TCPS (dashed line), and TCPS with SC514 100 µM (dotted line) following 24-h stimulation with TNF-α. ≠p < 0.008, *p < 0.05.
