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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Cell Transplant. 2009;18(3):261–273. doi: 10.3727/096368909788534979

Figure 5.

Figure 5

Matrix embedding attenuates induction of fractalkine mRNA expression in EC. Fractalkine mRNA expression was normalized to GAPDH mRNA expression. (A) The fractalkine/GAPDH ratio for all treatment conditions was expressed as fold induction or repression of the fractalkine/GAPDH ratio determined for stimulation of MEEC with 100 U/ml TNF-α and IFN-γ for 4 h. Data are presented as mean ± SD from eight different experiments. (B) Cytokine stimulation induces significantly lower endothelial fractalkine expression when EC are matrix embedded. Intracellular expression of fractalkine on EC was analyzed by flow cytometry. Ten thousand cells were analyzed. Data are expressed as mean percentage ± SD of EC from eight different experiments at each time point; MEEC secrete lower amounts of soluble fractalkine. (C) Detection of secreted fractalkine levels in supernatants of cytokine-stimulated EC was performed with ELISA. Each value represents mean ± SD from eight different experiments for the denoted sampling period of 4 h. (D) Spearman correlation of soluble fractalkine levels (sCX3CL1) and percentage of EC expressing fractalkine intracellular (CX3CL1+-EC). Area of the density ellipse represents the 95% confidence interval.