Figure 6.

NK cell binding to EC is reduced when EC are matrix embedded. EC were grown to confluence on polystyrene plates or in Gelfoam matrices, activated with 100 U TNF-α and IFN-γ for 20 h. (A) Attachment of NK cells to cytokine-stimulated EC was visualized via immunofluorescence microscopy (10×) and quantified with attachment of ⁵¹Cr-labeled NK cells (0.5 × 10⁶/well). Labeled cells were resuspended in medium alone or medium containing 20 µg/ml anti-CX3CR1 antibody and added to the EC under gentle rocking conditions (10 cycles/min). After 30 min the medium was decanted and the wells were gently washed. Adherent cells were lysed by treating with 1% Triton in PBS. (B) Total binding was determined by measuring individual well-associated cpm using a gamma counter. Each value represents mean ± SD from eight different experiments.