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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Exp Eye Res. 2010 Feb 17;90(5):617–623. doi: 10.1016/j.exer.2010.02.006

Figure 1.

Figure 1

Effect of variant glucose on IL-1β: Retinal pericytes were incubated with 20mM glucose for 2 or 4 days, followed by 5mM glucose for 4 or 8 additional days. The cells incubated in continuous 5mM or 20mM glucose served as controls. (a) IL-1β mRNA was determined by RT-PCR method using the TaqMan Assays on Demand (ABI), and (b) its concentration was quantified by ELISA using a kit from R& D Systems. Each measurement was made in duplicate using 3 or more different cell preparations. The values are represented as mean ±SD, and the numbers obtained in continuous 5mM are considered as 100%. 5mM or 20mM= exposure of cells to continuous 5mM glucose or 20mM glucose; 2d-4d=2days of 20mM followed by 4days of 5mM glucose; 4d-4d= 4days of 20mM followed by 4days of 5mM glucose, and 4d-8d= 4days of 20mM followed by 8days of 5mM glucose. *P<0.05 compared to continuous 5mM glucose, and #P<0.05 compared to continuous 20mM glucose.