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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Prostate. 2010 May 15;70(7):765–776. doi: 10.1002/pros.21109

Fig. 1. Treatment of 5-aza-dC by limited demethylation approach preferentially demethylated specific CpG dinucleotide in PMP24 promoter CGI.

Fig. 1

A: The organization of PMP24 promoter CGI. The major 5-aza-dC-hypersensitive site at intronic CpG 1 and 2 that corresponding to CG29 and CG30 is marked with a line. B: Relative PMP24 expression levels, as determined by real-time RT-PCR, among the normal and malignant prostate cell lines, which correlated with the extent of promoter methylation [25]. C: Treatment with 5-aza-dC reactivated PMP24 gene expression in a dose-dependent manner, with 0.1 µM the lowest concentration causing gene reactivation (n=4). D: Using the limited demethylation approach, we found one major region in PMP24 CGI that is hypersensitive to 5-aza-dC demethylation after PC-3 cells were treated briefly with a very low dose of 5-aza-dC. Figure 1A and 1D represent the same region with the same scale.