Figure 1.
Set-up of experiment.
The organ bath is composed of an inside small chamber and an outside chamber. The Krebs solution contained in the small chamber was maintained constant at 37 °C by circulating water in the big chamber using a heating machine. The intestinal segment was placed in the small chamber containing Krebs solution. The volume was applied by a pump and the stretch ratio regulated by a micromanipulator. The pressure probe was used to measure the pressures. The diameter changes of the intestinal segments were videotaped through a stereomicroscope.