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. Author manuscript; available in PMC: 2011 Apr 20.
Published in final edited form as: Acc Chem Res. 2010 Apr 20;43(4):551–563. doi: 10.1021/ar900255c

Chart 3.

Chart 3

Structures of an example of G-2 modified nucleotides. (a) Structures of the nucleotide reversibleterminators, 3′-O-N3-dATP, 3′-O-N3-dCTP, 3′-O-N3-dGTP, 3′-O-N3-dTTP. (b) Structures of cleavablefluorescent dideoxynucleotide terminators ddNTP-N3-fluorophores, with the 4 fluorophores having distinctfluorescent emissions: ddCTP-N3-Bodipy-FL-510 (λabs (max) = 502 nm; λem (max) = 510 nm), ddUTP-N3-R6G (λabs (max) = 525 nm; λem (max) = 550 nm), ddATP-N3-ROX (λabs (max) = 585 nm; λem (max) = 602 nm), and ddGTP-N3-Cy5 (λabs (max) = 649 nm; λem (max) = 670 nm).