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. Author manuscript; available in PMC: 2011 Apr 20.
Published in final edited form as: Acc Chem Res. 2010 Apr 20;43(4):551–563. doi: 10.1021/ar900255c

Figure 1.

Figure 1

DNA immobilization on a surface with click chemistry. An amino modified glass surface isreacted with a linker containing an NHS-ester on one end and an alkyne on the other to functionalizethe surface (step 1). Azide labeled PCR product is then attached to the surface using click chemistry(step 2). The unattached DNA strand is removed under alkaline condition (step 3). A loop DNA primeris ligated to the single-stranded DNA template (step 4). DNA extension reaction is carried out toidentify the sequence of the template (step 5). The sequence of the loop primer is shown in (A).