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. Author manuscript; available in PMC: 2011 Apr 20.
Published in final edited form as: Acc Chem Res. 2010 Apr 20;43(4):551–563. doi: 10.1021/ar900255c

Figure 3.

Figure 3

DNA sequencing by synthesis with cleavable fluorescent nucleotide reversible terminators(CF-NRTs). DNA polymerase catalyzes the incorporation of a CF-NRT, which is complementary tothe next base on the unknown DNA template. After removing unincorporated nucleotide analoguesand other excess reagents, a fluorescence imager is used to identify the base just incorporated. Thenthe fluorophore and the 3′ capping moiety are chemically cleaved to reinitiate the DNA polymerasereaction for subsequent sequence determination.