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. Author manuscript; available in PMC: 2011 Apr 20.
Published in final edited form as: Acc Chem Res. 2010 Apr 20;43(4):551–563. doi: 10.1021/ar900255c

Figure 6.

Figure 6

Reaction scheme of pyrosequencing using 3′-capped NRTs. The incorporation of G-2Amodified nucleotides (3′-O-R-dNTPs) releases a pyrophosphate (PPi), which triggers enzymaticreactions to produce the light signal for sequence detection. Upon the removal of the reversiblecapping moiety (deprotection), the 3′-OH of the extended primer is regenerated to reinitiate thepolymerase reaction. The pyrosequencing reaction is repeated continuously to identify the sequenceof the target DNA template.