Figure 7. Mps1 is required for cell viability.

(a) HCT116 cells were treated with Mps1-IN-1 (2, 5, 10 µM) for 24, 48, 72, and 96 hours. Effects on cell proliferation were quantified by measuring fluorescence of Syto60 nucleic acid stain emitted at 695 nm (excitation 635 nm). Values were normalized to DMSO control for each time point. All graphics represent mean ± SD and were obtained from 4 independent experiments. (b) FACS profile of HCT116 cells treated with DMSO vehicle or Mps1-IN-1 (10 µM) for the indicated time points. (c) Colony outgrowth assay of HCT116 cells treated with DMSO vehicle or Mps1-IN-1 (2, 5, 10 µM). Cells were plated at a density of 200 cells/60-mm dish and harvested for crystal violet staining after 10 days. (d) Quantitation of colony outgrowth assays for HCT116 cells treated with DMSO vehicle or Mps1-IN-1 (2, 5, 10 µM). All graphics represent mean + SD and were obtained from 3 independent experiments (**p-value = 3.22e⁻⁰⁵, Student’s t test). (e) Immunoblot of PARP cleavage of HCT116 cells treated with Mps1-IN-1 at the indicated concentrations. Cell lysates were harvested after 24, 48, and 72 hours for immunoblot detections.