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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Feb;67(2):120–133. doi: 10.1002/cm.20429

Figure 7. Role of the WH2 domain in promoting nucleotide exchange on ADF/cofilin-bound ADP-actin monomers.

Figure 7

All assays contained 2 μM ADP-G-actin and 5 μM Cof1, except where noted. (A) Comparison of the effects of full-length wild type Srv2 protein (Srv2-WT) and profilin on rate of nucleotide exchange. (B) Comparison of the effects of Srv2-WT and mutant Srv2-97 and Srv2-98 proteins on rate of nucleotide exchange. (C) Comparison of the effects of different fragments of Srv2 (400 nM) on rate of nucleotide exchange. (D) Comparison of the abilities of wild type and mutant WH2 peptides to restore nucleotide exchange activity to full-length Srv2-98 protein. (E) Effects of different domains of Srv2, alone and in combination (400 nM or 4 μM, as indicated), on the nucleotide exchange rate of Cof1-bound ADP-G-actin. (F) Effects of yeast profilin (5 μM) on the nucleotide exchange rate of ADP-G-actin in the presence and absence of full-length Srv2-WT (400 nM) and/or Cof1 (5 μM).