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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Nat Immunol. 2010 Apr 11;11(5):404–410. doi: 10.1038/ni.1861

Figure 3.

Figure 3

M2 channel activity of influenza virus is required for inflammasome activation. Cells were infected with WT (A/Udorn) or M2del29-31 (A/Udorn) (a–d) virus. (a) Supernatants from BM DCs infected at indicated MOIs were collected 24 h after infection and analyzed for IL-1β, IL-18, IL-12p40, and TNF-α by ELISA. (b) Supernatants and cell extracts from infected BMM were collected at the indicated time points and analyzed for the presence of pro- and mature-caspase-1 by Western blotting. (c) Pro-IL-1β mRNA levels from infected BMM were assessed by RT quantitative PCR. (d) Supernatants and cell extracts from infected BMM were collected 24 h after infection and the presence of pro- and mature IL-1β were analyzed by Western blotting. Data represent the mean ± S.D, and are representative of at least three independent experiments.