Figure 8.
M2 channel-induced inflammasome activation requires acidified Golgi compartment. (a) BMM and BM DC were infected with wild-type or M2del29-31 A/Udorn virus. Six hours later, culture media were replaced with media with the indicated pH levels. Green arrows indicate cytosolic pH level of 7.2. (b) RAW264.7 cells transduced with E5 WT, E5 Q17G, or GFP-expressing retroviruses were infected with A/PR8 at the indicated MOIs. Supernatants were analyzed for IL-1β by ELISA 24 h after infection. Data represents the mean ± S.D. Similar results were obtained from at least three separate experiments.