Fig. 5.

Mean peptide levels of NPY (a), des-α-MSH (b), and β-END (c) in the ARC of normal, lean, and lean leptin-treated (50 μg/h) OVX ewes. All data are presented as means ± SEM. d HPLC/EIA profile of MSH standards, des-α-MSH, act-α-MSH and di-act-α-MSH as well as representative HPLC/EIA profiles in the ARC and cerebellum. e Analysis of mean LH concentration (ng/ml), LH pulse amplitude (ng/ml), LH interpulse interval (min) and LH pre-pulse nadir (ng/ml) presented as means ± SEM. * p < 0.05 and ** p < 0.01 compared to ewes of normal body weight; ‸ p < 0.05 and ‸‸ p < 0.01 compared to pre-treatment −4 to 0 h LH values; ^† p < 0.05 compared to lean −4 to 0 h LH interpulse interval.