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. 2010 Apr 20;4(4):e664. doi: 10.1371/journal.pntd.0000664

Figure 2. Analytical specificity of PCR followed by 6% polyacrylamide gel electrophoresis with silver staining and ELISA (optical density, OD) for purified DNA from adult S. magrebowiei (lane 2), S. rhodaini (lane 3), S. japonicum (lane 4), S. intercalatum (lane 5), S. haematobium (lane 6), S. bovis (lane 7) and S. mansoni (lane 8) worms.

Figure 2

Lane 1, PCR negative control. The genus specificity of the Schistosoma PCR-ELISA system was demonstrated by the amplification of the 121-bp tandem repeat DNA sequence in all samples with the same set of primers. Absorbance readings were also consistent with the positive control (S. mansoni DNA).