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. 2010 Apr 20;5(4):e10243. doi: 10.1371/journal.pone.0010243

Figure 5. Two independent E2F4-siRNA reversed the anti-proliferative effect of capsaicin in H69 cells.

Figure 5

(A) The transfection of siRNA and subsequent BrdU assay was performed as described in “Materials and Methods.” (B) The results obtained by BrdU assay are confirmed with PCNA ELISA assay. Two independent E2F4-siRNA were transfected as described in “Materials and Methods.” PCNA assays were then performed to assess the levels of E2F4-siRNA on the anti-proliferative activity of capsaicin. (C) Western blotting analysis indicated that E2F4 levels are suppressed upon siRNA transfection. GAPDH was used as the loading control for the western blotting experiments, and the results were quantitated by densitometric analysis. Values indicated by an “*” are statistically significant.