Fig. 2.

Schematic of the perfusion bioreactor employed with microintegrated constructs. 13 mm diameter construct discs (a) were placed between O-rings (b) and a support screen (c) of in-line filter holders (d) followed by perfusion at 0.5 mL/min with a multi-channel peristalic pump (e). Each construct was placed in its own loop consisting of a 32 mL media bag (f), silicone tubing gas exchanger (g) and syringes for media exchange (h).