. Author manuscript; available in PMC: 2010 Aug 1.

Published in final edited form as: Nat Struct Mol Biol. 2010 Jan 10;17(2):159–164. doi: 10.1038/nsmb.1737

Table 2.

Substrate Dependence for Alda-1 Activation^a

Substrate	V_max^(app) (min⁻¹)	K_Act^(app) (μM)	V_max/V_o
100 μM Acetaldehyde	107 +/− 12	0.98 +/− 0.20	1.8 +/− 0.1
100 μM Propionaldehyde	78.5 +/− 9.6	5.1 +/− 1.2	1.7 +/− 0.1
100 μM Butyraldehyde	86.6 +/− 3.1	1.8 +/− 0.5	1.3 +/− 0.1

Data were fit to the non-essential activator expression (see Methods), where V_o is the initial velocity for the reaction in the absence of activator, V_max is the maximal velocity and K_Act is the concentration of activator required for half-maximal activation