Table 4.
Esterase Activity Activation Constants for ALDH2 and ALDH2*2a
| Constant | ALDH2 | ALDH2 (0.5 mM NAD+) | ALDH2*2 | ALDH2*2 (1. 0 mM NAD+) | ALDH2*2 (50 μM Alda-1) |
|---|---|---|---|---|---|
| Vo (min−1) | 24.9 +/− 2.0 | 96.3 +/− 2.2 | 0.40 +/− 0.03 | 1.36 +/− 0.28 | 0.64 +/− 0.19 |
| Vmax (min−1) | 181 +/− 6.8 | 248 +/− 22 | 2.3 +/− 0.2 | 14.7 +/− 1.3 | 49.5 +/− 4.3 |
| KAct(app) (μM) | 3.4 +/− 0.5 | 2.6 +/− 0.1 | 16.1 +/− 5.8 | 11.2 +/− 1.3 | 2,820 +/− 330 |
| Vmax (min−1) | -- | 242 +/− 14 | -- | -- | -- |
| Ki(app) (μM) | -- | 328 +/− 24 | -- | -- | -- |
a
Data were fit to the non-essential activator expression (see Methods), where Vo is the initial velocity for the reaction in the absence of activator, Vmax is the maximal velocity and KAct is the concentration of activator required for half-maximal activation and Ki is the concentration required for half-maximal inhibition.