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MALDI-TOF mass spectra of the chymotryptic digest of β-LG. (A). control; (B). β-LG incubated with LA (d0:d5 = 1:1), Fe(II) and Asc; (C). β-LG incubated with LA (d0:d5 = 1:1), Fe(II) and Asc with NaBH4 reduction. 832.5/837.6, 848.5/853.6 and 866.5/871.6 are modified HIRL at H146 by d0-/d5-KODDE, d0-/d5-EKODE and d0-/d5-DHKODE, respectively, through Michael addition. 834.6/839.6, 850.6/855.6 and 868.6/873.6 are reduced forms of above products, respectively.
