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. Author manuscript; available in PMC: 2011 Apr 27.
Published in final edited form as: Biochemistry. 2010 Apr 27;49(16):3381–3392. doi: 10.1021/bi100065r

Figure 3.

Figure 3

(A) Typical ITC dataset for Rsn5-NPF1 binding to EHD1 EH. The protein in the calorimeter cell was 50 μM and the peptide titrant was 0.5 mM. (B) Typical ITC dataset for Rfp2-NPF2 binding to EHD1 EH. The protein was 200 μM and the peptide 2 mM. Both experiments were carried out in 25 mM MOPS, 1 mM CaCl2 at 20°C.