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(A) Affinity of the first 4 NPF motifs of Rabenosyn-5 for EHD1 EH in 25 mM MOPS pH 7.0, 1 mM CaCl2, 20°C measured by ITC. (B) Systematic conversion of Rsn5-NPF3 into a high affinity peptide by systematic replacement of neutral residues with acidic residues in the positions following the NPF sequence. Error bars represent the standard deviation between two measurements.
