Table 4.1.

Reagents for iMMEC culture

Reagent	Source	Stock	Storage
Collagenase A	Roche	1% in H2O	−20°
DNAse	Sigma	0.4 mg/ml in PBS	−20°
EGF	Sigma	10 μg/ml in PBS	4°
Fetuin	Sigma	2 mg/ml in 20% FBS	4°
F12	Invitrogen		4°
FBS	Invitrogen		−20°
Hydrocortisone	Sigma	1 mg/ml in EtOH	−20°
Insulin	Sigma	10 mg/ml in 25 mM HEPES, pH 8.2	4°
Matrigel, growth factor reduced	BD		−20°
Pen/Strep	Invitrogen	10,000 units	−20°
Prolactin	Sigma		4°
Prolong Antifade	Molecular Probes		−20°
Trypsin, regular strength	Invitrogen	0.05%	−20°
Trypsin, lower strength	Clonetics	0.025%	−20°