Table 4.2.

Medium recipes for iMMECs

Component	2× hormone plating medium	Reduced-FBS growth medium	Regular growth medium	Cloning medium	Differentiation medium
F12	250 ml	500 ml	500 ml	50 ml	500 ml
FBS	No FBS! (already in fetuin-serum solution)	25 ml (5% final)	50 ml (10% final)	10 ml (20% final)
EGF (10 μg/ml stock in PBS	250 μl (10 ng/ml final)	250 μl (5 ng/ml final)	250 μl (5 ng/ml final)	25 μl (5 ng/ml final)
Hydrocortisone (1 mg/ml stock in EtOH)	500 μl (2 μg/ml final)	500 μl (1 μg/ml final)	500 μl (1 μg/ml final)	50 μl (1 μg/ml final)	500 μl (1 μg/ml final)
Insulin (10 mg/ml stock)	250 μl (10 μg/ml final)	250 μl (5 μg/ml final)	250 μl (5 μg/ml final)	25 μl (5 μg/ml final)	250 μl (5 μg/ml final)
Prolactin					1.5 mg (3 μg/ml final)
Pen/Strep (10,000 units)	5 ml	5 ml	5 ml	0.5	5 ml