Table 4.3.

Useful antibodies and fluorescent reagents for iMMEC analysis

Antibody or stain	Purpose	Source
Activated (cleaved) caspase-3	Apoptosis marker	Cell signaling
β-casein	Milk protein	Santa Cruz
β-catenin	Cell–cell junctions	Zymed
Cytokeratin 5/6	Myoepithelial cell marker	Covance
Cytokeratin 8	Luminal cell marker	Abcam
Cytokeratin 14	Myoepithelial cell marker	Covance
DAPI	Nuclear counterstain	Sigma
E1A	Immortalization marker	Oncogene
E-cadherin	Cell–cell junctions	RDI
Ep-CAM	Epithelial cell marker	Santa Cruz
ER-α	Hormone receptor	Santa Cruz
Occludin	Cell–cell junctions	Zymed
p53 (for p53DD)	Immortalization marker	Oncogene
Smooth muscle actin (SMA)	Myoepithelial cell marker	Sigma
Vimentin	Myoepithelial cell marker	Santa Cruz
ZO-1	Cell–cell junctions	Zymed