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. Author manuscript; available in PMC: 2011 Apr 16.
Published in final edited form as: J Mol Biol. 2010 Feb 23;397(5):1144–1155. doi: 10.1016/j.jmb.2010.02.029

Figure 1. TEM of duplex mtRIs from sucrose gradient-purified mitochondria.

Figure 1

Panels A, B, D and E from HEK293 cells, panel C from mouse liver. Scale bars represent 200 nm. Interpretations alongside show duplex segments delineating an entire 16 kb circle in black, with the alternate branch of replication bubbles in red. A, B, D: examples of fully duplex θ-forms seen without (A, B) or with (C, D) formamide spreading. E: formamide spreading after RNase H treatment revealed extensive single-stranded regions (orange). For further examples see Suppl. Fig. 1, plus full data compiled in Table 1.