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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: J Neurosci Res. 2009 Sep;87(12):2632–2644. doi: 10.1002/jnr.22103

Fig. 8.

Fig. 8

The transcriptional up-regulation of zif268, Arc, and bdnf exon 4 requires prolonged synaptic activation by bicuculline. DIV 3 or 14 neurons were treated with bicuculline (50 uM) and analyzed for p-ERK and p-CREB (a) 30 min after stimulation (n=3). For b and c, the transcription of zif268, Arc, and Bdnf exon 4 was analyzed by RT-PCR. Samples were collect 1 hr (b) or 4 hrs (c) after bicuculline stimulation (n=4). RT-PCR did not reveal any transcriptional up-regulation 1 hr after bicuculline stimulation. The representative Western blots or RT-PCR results are displayed in the left panels. The quantifications are shown in the right panels (average +/1 SD; *:p<0.05). The development stages (DIV 3 and DIV 14) and treatments are indicated in the figure.