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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: J Biomol Screen. 2009 Sep 17;14(9):1045–1053. doi: 10.1177/1087057109343120

FIG. 4.

FIG. 4

(A) Dependence of perinucleolar compartment (PNC) prevalence on camptothecin concentration for manual scoring with a 60× objective compared to that determined by the IN Cell Analyzer 1000 MTA algorithm (normalized to vehicle-treated cells). The calculated EC50% values for camptothecin were 419 nM and 152 nM for manual counts and automated counts, respectively. (B) Dependence of PNC prevalence on actinomycin D concentration compared to camptothecin by the IN Cell Analyzer 1000 MTA algorithm. PNC prevalence determination by the algorithm does not show dependence on bleomycin concentration (a highly cytotoxic non-PNC-reducing compound). The EC50% value for actinomycin D was 133 pM (n=4 and error bars = SD).