Table 1.
Strains and plasmids
| Strain (gene name*) | Strain† | Description |
|---|---|---|
| P. aeruginosa PAO1-derived strains | ||
| PAO1 | DH20 | P. aeruginosa WT |
| PA1997 : : Tn | PAO1 transposon mutant 478‡ | |
| PA1998 : : Tn (dhcR) | PAO1 transposon mutant 3 | |
| ΔPA1999–PA2000 (dhcAB) | MJ99 | Clean PA1999–PA2000 deletion |
| PA2001 : : Tn (atoB) | PAO1 transposon mutant 59 | |
| PA5384 : : Tn | PAO1 transposon mutant 47947 | |
| PA5385 : : Tn (cdhB) | PAO1 transposon mutant 10906 | |
| PA5386 : : Tn (cdhA) | PAO1 transposon mutant 52057 | |
| PA5387 : : Tn (cdhC) | PAO1 transposon mutant 14404 | |
| PA5388 : : Tn | PAO1 transposon mutant 37920 | |
| P. aeruginosa PA14-derived strains | ||
| PA14 | DH122 | P. aeruginosa WT |
| ΔgbcA-B | DH802 | PA5410–PA5411 deletion (Wargo et al., 2008) |
| ΔdgcA | DH848 | PA5398 deletion (Wargo et al., 2008) |
| PA1998 : : Tn (dhcR) | PA14 transposon mutant 26061§ | |
| PA1999 : : Tn (dhcA) | PA14 transposon mutant 30401 | |
| PA2000 : : Tn (dhcB) | PA14 transposon mutant 40012 | |
| ΔPA1999–PA2000 (ΔdhcAB) | MJ98 | Clean PA1999–PA2000 deletion |
| PA5385 : : Tn (cdhB) | PA14 transposon mutant 27032 | |
| ΔPA5386–PA5387 (ΔcdhCA) | MJ262 | In-frame PA5386–PA5387 deletion |
| ΔPA5388 | MJ192 | Clean PA5388 deletion |
| ΔPA5389 (cdhR) | MJ11 | Clean PA5389 deletion |
| Plasmids | ||
| pUCP22 | High-copy Pseudomonas stabilized vector; GmR (Schweizer, 1991) | |
| pEX18-Gm | Integrating vector in P. aeruginosa; GmR (Schweizer, 1993) | |
| pMQ30 | Suicide vector; GmR (Shanks et al., 2006) | |
| pMQ80 | High-copy Pseudomonas/yeast shuttle vector (Shanks et al., 2006) | |
| pMW5 | pUCP22 containing lacZYA from pRS415 (Wargo et al., 2009) | |
| pMW19 | 6×His-tagged PA5389 in pMQ80 | |
| pMW34 | PA1999 promoter fusion in pMW5 | |
| pMW37 | 6×His-tagged PA1998 in pMQ80 | |
| pMW40 | PA1998 deletion plasmid | |
| pMW41 | PA1999–PA2000 deletion plasmid | |
| pMW46 | PA5388 promoter fusion in pMW5 | |
| pMW50 | 6×His-tagged PA1999 and untagged PA2000 in pMQ80 | |
| pMW56 | PA5388 deletion plasmid | |
| pMW79 | PA14 genomic clone containing the PA5388–PA5384 region | |
| pMW85 | PA5389 deletion plasmid | |
| pMW89 | Subclone of pMW79 containing only the PA5388–PA5384 region | |
| pMW91 | PA5385 and partial PA5384 sequence in pUCP22 | |
| pMW95 | 6×His-tagged PA5386 and untagged PA5385 in pMQ80 | |
| pMW96 | PA5388 coding sequence in pMQ80 | |
| pMW99 | PA5386–PA5387 deletion plasmid | |
| pMW102 | PA5386 and PA5385 coding region subcloned from pMW79 |
*Gene names refer to the P. aeruginosa database nomenclature or are specified in this report.
†Strain numbers for non-transposon strains refers to the database designation used in our laboratory to facilitate strain requests.
‡PAO1 transposon mutants are numbered according to the two-allele library (Jacobs et al., 2003). All PAO1 transposon strains are tetracycline-resistant.
§PA14 transposon mutants are numbered according to the database identification numbers (Liberati et al., 2006). All PA14 strains are gentamicin-resistant.