Rats were microinjected with gp120 into the superior colliculus. Three days later, rats were sacrificed and gp120 immunoreactivity was analyzed in serial sections from the visual cortex. A, example of a section stained for neurofilament (green) and DAPI (blue). B, example of a section stained for gp120 (red) and DAPI (blue). C, merge of A and B. D, example of a section stained for GFAP (red), DAPI (blue) and gp120 (green). E, example of a section in which the primary antibody for gp120 was omitted. F, section in E was counterstained for DAPI (blue). Images are representative of ten sections per animal. Scale bar= 100μm.