Fig. 2.

Binding affinity is higher for muscimol and GABA at α₆β₃γ₂ than α₁β₃γ₂. (A) Saturation binding isotherms for α₁β₃γ₂ and α₆β₃γ₂ receptors obtained by using increasing concentrations of [³H]muscimol. Data points show the means ± SEM from a single experiment, with each concentration performed in triplicate. This experiment was performed five times, with similar results. (B) Shows a Scatchard plot from the same experiment from (A). Data points are the means of triplicate measurements. (C) An inhibition curve showing displacement of 10 nM [³H]muscimol with increasing concentrations of GABA. This figure represents the means ± SEM from a single experiment, with each point performed in triplicate. This experiment was performed three times with similar results.