Figure 4.
Imaging and therapy of schwannomas in vivo. Five million HEI-193-FC cells were implanted subcutaneously in nude mice. When tumors reached 100 mm3 in volume (day 7, tumor volume set at 100% volume for each tumor), as calculated by caliper measurements, mice were injected with 107 tu HGP0-ICE-lacZ or HGP0-lacZ vector on days 7, 10 and 13 (arrows). Tumor growth was monitored overtime by both in vivo bioluminescence imaging after injection of d-luciferin and acquiring photon counts using a CCD camera with quantitation of signal using CMIR image program (a, b), or by caliper measurements (c). A clear decrease in Fluc activity was observed up to day 20 in tumors injected with HGP0-ICE-lacZ amplicon vector consistent with a decrease in tumor size as determined by caliper measurements as opposed to tumors injected with HGP0-lacZ, which continued to increase in size (level of significance between untreated and treated tumors on day 16, *P < 0.002). Values shown as mean±s.d.