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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Gastroenterology. 2007 Jul 25;133(4):1316–1326. doi: 10.1053/j.gastro.2007.07.020

Figure 6. The G85V and G591D mutations lead to defective localization of ATP7B to the endoplasmic reticulum.

Figure 6

HEK293T cells were transfected with ATP7B-Flag (A–C and J–L), ATP7B-G85V-Flag (D–F and M–O) or ATP7B-G591D-Flag (G–I and P–R) and analyzed using antibodies against ATP7B (A, D, G, J, M, and P; green), p230 (B, E, and H; red) or Calreticulin (K, N, and Q; red), visualized by Alexa488 conjugated donkey anti-rat, Alexa568 conjugated donkey anti-mouse, Alexa568 conjugated donkey anti-rabbit IgG respectively. Overlap in staining is depicted in yellow in the overlay images (C, F, I, L, O, and R).