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. Author manuscript; available in PMC: 2010 Apr 21.
Published in final edited form as: Oncogene. 2009 Nov 30;29(9):1270–1279. doi: 10.1038/onc.2009.427

Figure 6.

Figure 6

Proliferative activity assay by blastocyst outgrowth in vitro. Blastocysts, (a) wild-type and (b) hausp knockout embryos, were collected from hausp heterozygote intercross at day E3.5 and cultured individually in vitro for 5 days. (c) Wild-type blastocysts showed prominent outgrowth of the embryonic cells (dotted circle) from the inner cell mass of the blastocyst on day 5. Trophoblastic giant cells are indicated by arrows. (d) Hausp knockout embryo showed virtually no outgrowth of the embryonic cells (dotted circle), the majority of the surviving cells were trophoblastic giant cells (arrows).