Immunoreactions for syndecan-1 (A–C), perlecan (D–F), agrin (J–L), and glypican-3 (M–O) in normal and diseased human liver. G–I show merged images of A–C and D–F. Left column shows normal liver tissue; middle column shows cirrhotic tissue; and right column shows HCC tissue. Thin arrows, blood vessels; thick arrows, bile ducts; arrowheads, HCC microvessels. Bars: A–O = 200 μm; insets = 50 μm. Bar in A applies to A–I; bar in J applies to J–O.