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Solubilization of flavoHb inclusion bodies produced in automatic fermenter (panel A) and shake flask (panel B). Same amounts of IBs were re-suspended in 40 mM Tris/HCl, pH 8.0 buffer containing different non denaturing agents. Same volumes of solubilized proteins were analysed by SDS-PAGE. Black star indicates that the loaded sample corresponds to one tenth of the other samples.
