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Cell surface binding of VACV IFNα/βBP and its VARV and MPXV orthologues. A) Anti-V5 immunofluorescent staining of nonpermeabilized HeLa cells incubated with supernatants containing the indicated V5-tagged recombinant proteins. Cell nuclei were visualized using DAPI. B) Confocal microscopy of cells incubated as before, showing plasmatic membrane localization of the indicated proteins.
