Figure 7. 20-HETE increases murine airway sensitivity to carbachol.

Lung slices from naïve mice were incubated over-night with 20-HETE (10 and 1 ng/mL). (A) Airways were contracted to carbachol (CCh) to create concentration-response curves. Incubation with 20-HETE (B) decreased log EC₅₀ values; but, (C) had no effect on maximum contraction compared to control slices. Data expressed as mean ± SEM shown; * P≤0.05 and ** P≤0.01 vs control (exact P values shown). At least 8 airways were used in each group, and never more than 12.