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. 2010 Apr 20;5(4):e10215. doi: 10.1371/journal.pone.0010215

Figure 1. Genotyping of PPAR γ flfl; Villin Cre+ (VC+) and Villin Cre- (VC-) control mice.

Figure 1

Conditional deletion of the PPAR γ gene via Villin Cre-mediated recombination was examined in mouse intestine by PCR analysis. The floxed (fl) allele at 275 bp and the null allele at 400 bp. (A) Left to right: depicts fl/fl in homogenized whole duodenum, jejunum, ileum, cecum and colon without recombination (VC-) (lanes 1, 3, 5, 7 and 9) or with recombination (VC+) (lanes 2, 4, 6, 8 and 10). (B) Left to right: depicts fl/fl in epithelial cells isolated from duodenum, jejunum, ileum, cecum and colon without recombination (VC-) (lanes 1, 3, 5, 7 and 9) or with recombination (VC+) (lanes 2, 4, 6, 8 and 10).