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. 2010 Apr 20;5(4):e10260. doi: 10.1371/journal.pone.0010260

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Tsutsumi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Coimmunoprecipitation of Cdk5/p35 with AATYK1A in HEK293 cells. AATYK1A-Flag was coexpressed with p35 and/or Cdk5 in HEK293 cells and immunoprecipitated from cell lysates using the anti-Flag antibody. p35 and Cdk5 were detected in the anti-Flag immunoprecipitates by immunoblotting using anti-p35 and anti-Cdk5 antibodies. (B) Coimmunoprecipitation of Cdk5/p35 from mouse brain extracts using the anti-AATYK1 antibody. AATYK1 was immunoprecipitated from a mouse brain extract (10 weeks). p35 and Cdk5 were detected in the AATYK1 immunoprecipitates using the anti-p35 and anti-Cdk5 antibodies.