FIG. 7.

Normal leptin receptor and IRS2 expression in the hypothalami of wild-type (WT) and VIP knockout (KO) mice. A: Representative Western blots of three wild-type (1–3) or VIP knockout (1–3) mice hypothalami. The tissue extracts were probed with specific antisera for the leptin receptor (Ob-Rb), IRS2, and β-actin as a loading control. For the Ob-Rb blot, a plasma membrane extract was used, whereas cytoplasmic tissue extracts were used for the IRS2 and actin blots. B–E: Mean ± SEM of the relative band intensities for the specific Western blots measured as relative absorbance units minus background absorbance per square pixel (AU-B/px²). F: Mean food intake (g) in wild-type and VIP knockout mice. G: Mean body weight of wild-type and VIP knockout mice. Values are expressed as means ± SEM.